KPNB1 anticorps (AA 8-876)

N° du produit ABIN7602502

L’anticorps Lapin Polyclonal anti-KPNB1 a été validé pour WB, ELISA, FACS, IF et ICC. Il convient pour détecter KPNB1 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour KPNB1 anticorps (AA 8-876) (ABIN7602502)

Antigène: KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp KPNB1 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-KPNB1 anticorps

Épitope: AA 8-876

Fonction: Anti-KPNB1 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-KPNB1 Antibody Picoband® (ABIN7602502). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human KPNB1 recombinant protein (Position: E8-A876).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ayala-Madrigal, M. L., Doerr, S., Ramirez-Duenas, M. L., Hansmann, I. Assignment of KPNA4 and KPNB1 encoding karyopherin alpha 4 and beta 1 to human chromosome bands 11q22 and 17q21 respectively, by in situ hybridization. Cytogenet. Cell Genet. 89: 258-259, 2000. 2. Bayliss, R., Littlewood, T., Stewart, M. Structural basis for the interaction between FxFG nucleoporin repeats and importin-beta in nuclear trafficking. Cell 102: 99-108, 2000. 3. Caudron, M., Bunt, G., Bastiaens, P., Karsenti, E. Spatial coordination of spindle assembly by chromosome-mediated signaling gradients. Science 309: 1373-1376, 2005.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur KPNB1

Antigène: KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))

Autre désignation: KPNB1

Sujet:

Synonyms: Bone morphogenetic protein 15, BMP-15, Growth/differentiation factor 9B, GDF-9B, BMP15, GDF9B

Background: Importin subunit beta-1 is a protein that in humans is encoded by the KPNB1 gene. Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene.

Poids moléculaire: 97 kDa

ID gène: 3837

UniProt: Q14974

Pathways: Protein targeting to Nucleus